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. 2020 Sep 10;10(3):2045894020948470. doi: 10.1177/2045894020948470

Fig. 6.

Fig. 6.

CM collected from normal and IPAH PAEC stimulates PASMC proliferation. (A) Schematic diagram (a) showing how the CM was collected for MTT experiments from normal (Nor-PAEC) and IPAH PAEC (IPAH-PAEC) and applied to PASMC isolated from normal subjects (normal PASMC) and IPAH patients (IPAH-PASMC) to test the effects of PAEC-CM on PASMC proliferation. Summarized data (b, means ± SE) showing MTT activity in normal PASMC (Nor-PASMC) and IPAH-PASMC cultured for 48 h in Nor-PAEC-CM or IPAH-PAEC-CM collected 24 or 48 h after incubation with normal or IPAH PAEC. *p < 0.05, **p < 0.01, ***p < 0.001 versus control (0.3% FBS basal medium, the dotted line); ###p < 0.001 versus Nor-PASMC. 0.3% FBS basal medium was used for the collection. (B) Summarized data (means ± SE) showing MTT activity in Nor-PASMC and IPAH-PASMC cultured for 48 h in 0.3% FBS basal medium or 10% FBS basal medium. ***p < 0.001 versus 0.3% FBS basal medium; #p < 0.05 versus Nor-PASMC. FBS: fetal bovine serum; IPAH: idiopathic PAH; MTT: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide; PAEC: pulmonary arterial endothelial cell; PASMC: pulmonary arterial smooth muscle cell.