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DNPH-based assays for PCO detection. Assays for the detection of PCO involve the derivatization with 2,4-DNPH, leading to the formation of a stable dinitrophenylhydrazone product. PVDF: polyvinylidene fluoride membrane; HRP: horseradish peroxidase; MALDI-TOF: matrix-assisted laser desorption/ionization time-of-flight; RRT: renal replacement therapy.
