Figure 3.

Comparison of the ‘open’ and ‘open-like’ crystal structures of Rad4–Rad23–DNA complexes. (A) Superposition of Rad4–Rad23–DNA crystal structures. The ‘open’ structures of WT Rad4 bound to 6–4PP (PDB ID: 6CFI) and of WT Rad4 tethered to CCC/GGG matched DNA (4YIR) are in red and green, respectively. The Δβ-hairpin3 and Δβ-hairpin2 mutants tethered to CCC/GGG matched DNA (cyan and magenta, respectively) show similar ‘open-like’ conformations. (B) Superposition of the DNA molecules extracted from the structures shown in (A). The difference in the DNA tails shown by the Δβ-hairpin2 (magenta) is partly due to the differences in crystal packing (Supplementary Discussion and Supplementary Figure S2).