Table 1.
Yeast strains used in this study
| No. | Name | Genotype | Source |
|---|---|---|---|
| 1991 | wt | MATa, ade2–1 his3–11 leu2–3 trp1–1 ura3–1 can1–100, UBR1::GAL1–10-Ubiquitin-M-LacI fragment-Myc-UBR1 (HIS3), leu2–3::pCM244 (CMVp-tetR’-SSN6, LEU2) x3 | S. Tanaka, J. F. Diffley, Nature Cell Biology. 4, 198–207 (2002). |
| 1993 | tof1Δ | 1991 + TOF1::hphMX | This study |
| 1528 | TOF1 | 1991 + TOF1::TOF1-codon-optimised-wildtype (NATNT2) | This study |
| 1549 | tof1 627 | 1991 + TOF1::tof1-codon-optimised-627–1238Δ (NATNT2) | This study |
| 1596 | tof1 762 | 1991 + TOF1::tof1-codon-optimised-762–1238Δ (NATNT2) | This study |
| 1589 | tof1 830 | 1991 + TOF1::tof1-codon-optimised-830–1238Δ (NATNT2) | This study |
| 1546 | tof1 997 | 1991 + TOF1::tof1-codon-optimised-997–1238Δ (NATNT2) | This study |
| 1537 | tof1 1182 | 1991 + TOF1::tof1-codon-optimised-1182–1238Δ (NATNT2) | This study |
| 2188 | TOF1 wt TAP | 1991 + endogenous TOF1 tagged with TAP (KanMX) | This study |
| 2182 | TOF1 TAP | 1991 + TOF1::tof1-codon-optimised-wildtype-TAP (KanMX) | This study |
| 2183 | tof1 627 TAP | 1991 + TOF1::tof1-codon-optimised-627–1238Δ-TAP (KanMX) | This study |
| 2184 | tof1 762 TAP | 1991 + TOF1::tof1-codon-optimised-762–1238Δ-TAP (KanMX) | This study |
| 2185 | tof1 830 TAP | 1991 + TOF1::tof1-codon-optimised-830–1238Δ-TAP (KanMX) | This study |
| 2186 | tof1 997 TAP | 1991 + TOF1::tof1-codon-optimised-997–1238Δ-TAP (KanMX) | This study |
| 2187 | tof1 1182 TAP | 1991 + TOF1::tof1-codon-optimised-1182–1238Δ-TAP (KanMX) | This study |
| 2288 | TOF1 wt mrc1–3FLAG | 1528 + Mrc1–3FLAG (NATNT2) | This study |
| 2191 | TOF1 wt-TAP mrc1–3FLAG | 2182 + Mrc1–3FLAG (NATNT2) | This study |
| 2192 | tof1 627-TAP mrc1–3FLAG | 2183 + Mrc1–3FLAG (NATNT2) | This study |
| 2194 | tof1 762-TAP mrc1–3FLAG | 2184 + Mrc1–3FLAG (NATNT2) | This study |
| 2195 | tof1 830-TAP mrc1–3FLAG | 2185 + Mrc1–3FLAG (NATNT2) | This study |
| 2196 | tof1 997-TAP mrc1–3FLAG | 2186 + Mrc1–3FLAG (NATNT2) | This study |
| 2197 | tof1 1182-TAP mrc1–3FLAG | 2187 + Mrc1–3FLAG (NATNT2) | This study |
| 2465 | GAL-HA-TOF1 wt | 1991 + TOF1:: GAL-S-HA-TOF1-codon-optimised-wt (TRP) | This study |
| 2466 | GAL-HA-tof1 627 | 1991 + TOF1:: GAL-S-HA-TOF1-codon-optimised-627–1238Δ (TRP) | This study |
| 2467 | GAL-HA-tof1 997 | 1991 + TOF1:: GAL-S-HA-TOF1-codon-optimised-997–1238Δ (TRP) | This study |
| 2468 | GAL-HA-tof1 1182 | 1991 + TOF1:: GAL-S-HA-TOF1-codon-optimised-1182–1238Δ (TRP) | This study |
| 2414 | wt TOF1 (diploid) | MAT a/alpha (diploid) homozygous: ade2–1 his3–11 leu2–3 trp1–1 ura3–1 can1–100, UBR1::GAL1–10-Ubiquitin-M-LacI fragment-Myc-UBR1 (HIS3), leu2–3::pCM244 (CMVp-tetR’-SSN6, LEU2) x3,heterozygousTOF1::tof1-codon-optimised-wildtype (NATNT2) | This study |
| 2420 | tof1 627 TAP top1-MYC (diploid) | MAT a/alpha (diploid) heterozygous: ade2–1 his3–11 leu2–3 trp1–1 ura3–1 can1–100, UBR1::GAL1–10-Ubiquitin-M-LacI fragment-Myc-UBR1 (HIS3), leu2–3::pCM244 (CMVp-tetR’-SSN6, LEU2) x3 TOF1::tof1-codon-optimised-627–1238Δ-TAP (KanMX) Top1–9MYC (hphMX) | This study |
| 2463 | tof1 997 TAP top1-myc (diploid) | MAT a/alpha (diploid) homozygous: ade2–1 his3–11 leu2–3 trp1–1 ura3–1 can1–100, UBR1::GAL1–10-Ubiquitin-M-LacI fragment-Myc-UBR1 (HIS3), leu2–3::pCM244 (CMVp-tetR’-SSN6, LEU2) x3, heterozygous TOF1::tof1-codon-optimised-997–1238Δ-TAP (KanMX) Top1–9MYC (hphMX) | This study |
| 2416 | tof1 1182 TAP top1-myc (diploid) | MAT a/alpha (diploid) homozygous: ade2–1 his3–11 leu2–3 trp1–1 ura3–1 can1–100, UBR1::GAL1–10-Ubiquitin-M-LacI fragment-Myc-UBR1 (HIS3), leu2–3::pCM244 (CMVp-tetR’-SSN6, LEU2) x3, heterozygousTOF1::tof1-codon-optimised-1182–1238Δ-TAP (KanMX) Top1–9MYC (hphMX) | This study |
| 1988 | csm3Δ | 1991 + CSM3:: NATNT2 | This study |
| 684 | tof1Δ rad9Δ | 1993 + rad9::NATNT2 | This study |
| 2405 | csm3Δ rad9Δ | 1988 + rad9::KanMX | This study |
| 1718 | TOF1 wt rad9Δ | 1528 + rad9::KanMX | This study |
| 1719 | tof1 627 rad9Δ | 1549 + rad9::KanMX | This study |
| 1777 | tof1 762 rad9Δ | 1596 + rad9::KanMX | This study |
| 1767 | tof1 830 rad9Δ | 1589 + rad9::KanMX | This study |
| 1722 | tof1 997 rad9Δ | 1546 + rad9::KanMX | This study |
| 1769 | tof1 1182 rad9Δ | 1537 + rad9::KanMX | This study |
| 1762 | TOF1 wt top2–4 pRS316 | 1528 + top2–4,pRS316 (URA) | This study |
| 1610 | tof1 627 top2–4 pRS316 | 1549 + top2–4,pRS316 (URA) | This study |
| 2290 | tof1 762 top2–4 pRS316 | 1596 + top2–4,pRS316 (URA) | This study |
| 1717 | tof1 830 top2–4 pRS316 | 1589 + top2–4,pRS316 (URA) | This study |
| 1763 | tof1 997 top2–4 pRS316 | 1546 + top2–4,pRS316 (URA) | This study |
| 1611 | tof1 1182 top2–4 pRS316 | 1537 + top2–4,pRS316 (URA) | This study |
| 1731 | wt pRS426-RFB | 1991 + pRS426–1xRFB (URA) | This study |
| 1730 | tof1Δ pRS426-RFB | 1993 + pRS426–1xRFB (URA) | This study |
| 1732 | TOF1 wt pRS426-RFB | 1528 + pRS426–1xRFB (URA) | This study |
| 1739 | tof1 627 pRS426-RFB | 1549 + pRS426–1xRFB (URA) | This study |
| 2333 | tof1 762 pRS426-RFB | 1596 + pRS426–1xRFB (URA) | This study |
| 1740 | tof1 830 pRS426-RFB | 1589 + pRS426–1xRFB (URA) | This study |
| 1733 | tof1 997 pRS426-RFB | 1546 + pRS426–1xRFB (URA) | This study |
| 1741 | tof1 1182 pRS426-RFB | 1537 + pRS426–1xRFB (URA) | This study |