Figure 2.

NTS is a novel oncogene in UM. (A, B) Colony formation analysis demonstrated that NTS-silenced cells exhibited fewer colonies and could be rescued by recombinant NTS supplementation in OCM1 and OCM1a cells. Data are presented as the mean ± SD and the differences between two groups were calculated by unpaired two-tailed Student's t-test. (C) Animal imaging system demonstrated the suppressive effects on tumor bioluminescent signals in NTS-silenced OCM1 cells in orthotopic xenografts. * P <0.05, ** P < 0.01. (D) Suppressive effects on tumor volume in NTS-silenced OCM1 cells in a subcutaneous xenograft model. * P < 0.05, ** P < 0.01. (E) The TCGA database of UM demonstrated prolonged survival time in patients with low NTS expression.