Figure 5.

Expression of the compensatory double-mutant G57C/C74G, but not of G57C, is lethal in vivo. (A) E. coli cells carrying pBAD plasmids as indicated were grown to OD₆₀₀ = 0.5 in a Tecan plate reader at 37°C before addition of 0.025% glucose. Time of addition is indicated (red arrow). (B) Same as in (A), but with arabinose induction (0.025%). (C) Ten-fold serial dilutions of bacterial cultures 30 min post-induction with either glucose or arabinose were spotted on LA-amp plates.