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. 2020 Nov 9;48(21):12085–12101. doi: 10.1093/nar/gkaa965

Figure 1.

Figure 1.

Time-dependent repression of DNA repair genes after B[a]P treatment in metabolically competent MCF7 breast cancer cells (A) and upon BPDE exposure in VH10tert cells (C) was measured by qPCR. Time-dependent repression of DNA repair proteins upon B[a]P treatment in MCF7 cells (B) and after BPDE exposure in VH10tert cells (D) was measured by immunoblotting. β-Actin was used as internal loading control, x-fold induction was measured densitometrically and is annotated under the respective blot. (E) Binding of MSH2/MSH6 to GT-mismatches was measured via EMSA. GC-oligonucleotides were included as specificity control; 1 = specific band, 2 = unspecific bands, 3 = unbound oligonucleotides, x-fold induction was measured densitometrically and is annotated under blot. (F) HR activity was analysed using a PCR-based HR activity assay. (A, C, F) Experiments were performed in triplicates and differences between treatment and control were statistically analyzed using Student's t test (non labeled = non significant, *P< 0.1 **P< 0.01, ***P< 0.001).