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. 2020 Nov 9;48(21):12085–12101. doi: 10.1093/nar/gkaa965

Figure 2.

Figure 2.

(A) Binding of E2F1 to the promoters of EXO1, MSH2, MSH6 and RAD51 was analysed by ChIP and subsequent qPCR 24, 48 and 72 h after B[a]P (1 μM) or BPDE (0.25 μM) treatment. The amount of the immunoprecipitated mRNA in the control was set to 1. Experiments were performed in triplicates and differences between treatment and control were statistically analyzed using Student's t test (non labelled = non significant, *P< 0.1 **P< 0.01, ***P< 0.001). (B) E2F1 activity was measured by EMSA 24 and 48 h upon B[a]P in MCF7 cells and upon BPDE in VH10tert cells; 1 = specific band, 2 = unspecific bands, 3 = unbound oligonucleotides, x-fold induction was measured densitometrically and is annotated under blot. (C) Time-dependent expression of E2F1, pRb and Rb was measured by immunoblotting in MCF7 and VH10tert cells upon B[a]P and BPDE treatment respectively. β-Actin was used as internal loading control.