Figure 3.

Comparative presentation of the most commonly used indel detection methodologies for genome editing. Methods shown are; next-generation sequencing (NGS), Sanger sequencing based methods via cloning (Topo TA) or sequence trace decomposition (TIDE/ICE), enzyme mismatch cleavage assay (EMC), restriction fragment length polymorphism assay (RFLP) and indel detection by amplicon analysis (IDAA). Approximate effort, time and cost (from low to high) required for completing the steps for each respective method from genome edited sample (light grey hexagon) to full insight is indicated at the bottom. QC indicates some of the necessary quality control steps required for NGS.