Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 24;48(21):12151–12168. doi: 10.1093/nar/gkaa1093

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — H3K4me is required for histone sumoylation. (A) Mutant set1Δ ulp2Δ strains carrying a YCplac33-ULP2 (URA3) cover plasmid and the indicated plasmids were grown on SD-Trp and SD+5-FOA at 30°C for 2–3 days. (B) Immunoblot assay of H3K4me3, me2, and me1 in the indicated strains. Anti-H3 blotting shows similar loading. (C and E) Immunoblot analysis of immunoprecipitated Flag-tagged H2B using anti-SUMO antibody in the indicated strains. Upper and lower panels show Flag-H2B-SUMO and Flag-H2B (loading control), respectively. Asterisks and open circles as in Figure 1A. (D) ChDIP analysis of Flag-H2B-SUMO for the strains in (C). Data represent mean ± SD of triplicates; *P <0.05 (Student's t test comparing to WT).