Figure 7.

The RT-qPCR analysis indicated effects of Rbs1 on the expression of yeast genes that are controlled by 3′-UTR NMD decay (A) but no influence of Rbs1 on the level of RPL28 pre-mRNA, a direct NMD target (B). RNAs that were isolated from the upf1Δ mutant and control strain (wt) that carried an empty vector [–] or [RBS1] plasmid were analyzed by RT-qPCR with probes that were specific to PGA1 mRNA, MSH4 mRNA, SPO16 mRNA, and CNN1 mRNA (A) or intron-containing RPL28 pre-mRNA and mature RPL28 mRNA (B). The levels of the tested mRNAs were normalized to SCR1 RNA and calculated relative to levels in the wt strain, which was set to 1. Bars represent the mean ± standard deviation of three independent experiments. P values were calculated using a two-tailed t-test.