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. 2020 Nov 9;48(21):12219–12233. doi: 10.1093/nar/gkaa1016

Figure 1.

Figure 1.

The Meiob N64I mutation causes stage XII-specific meiotic defects in males. (A) Testis size of 8-week-old MeiobN64I/+, MeiobN64I/N64I and MeiobN64I/− mice. (B, C) Reduced testis weight (B) and sperm count (C) in MeiobN64I/− males. n = 3, 8-week-old males. Data are presented as mean ± S.D., *P < 0.05 by Student's t-test. NS, non-significant. (D) The N64I substitution destabilizes the MEIOB protein in testes (postnatal day 20, P20). Band quantification was normalized to β-actin (ACTB). Western blotting was performed in triplicates. *P < 0.05. (E) Histological analysis of testes at P20 and P60. Stage XII tubules are shown. Apoptotic metaphase spermatocytes are marked by black arrowheads. Scale bar, 20 μm. (F) TUNEL analysis of testicular sections at P20. H3S10ph (Phosphorylated H3 at serine 10) is a metaphase marker. Scale bar, 20 μm. (G) Quantification of TUNEL-positive spermatocytes at P20. n, the number of TUNEL-positive stage XII tubules (TUNEL-negative stage XII tubules were excluded). Three mice per genotype were examined. *P < 0.05 by Student's t-test.