Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 17;48(21):e126. doi: 10.1093/nar/gkaa897

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 8. — DNA Ligase IV, XRCC4, and XLF suppress HDR, whereas ATM promotes HDR. (A) Percentage of cells with single DSBs repaired via HDR (% BFP+ cells) following DSB induction and subsequent repair in the presence of a BFP cDNA donor template in U2OS-CDDR-Clone-1 cells and clonal derivatives deleted of LIG4, XRCC4, or XLF. Data represent the mean of three independent experiments ± s.d. Statistical significance was determined using two-tailed Student's t-test; *P< 0.05, **P< 0.01, ***P< 0.001. (B) Representative FACS profiles of U2OS cells, U2OS-CDDR-Clone-1 cells, and clonal derivates of the latter deleted of LIG4, XRCC4, or XLF, following the induction of single DSBs and subsequent repair in the presence of a BFP cDNA donor template. (C) Percentage of cells with single DSBs repaired via HDR (% BFP+ cells) in U2OS-CDDR-Clone-1 cells and clonal derivatives deleted of ATM. Data represent the mean of three independent experiments ± s.d. Statistical significance was determined using two-tailed Student's t-test; *P< 0.05, **P< 0.01. (D) Percentage of cells with single DSBs repaired via HDR (% BFP+ cells) in U2OS-CDDR-Clone-1 cells treated with DMSO or increasing doses of ATM-specific inhibitor KU-55933. Data represent the mean of three independent experiments ± s.d. Statistical significance was determined using two-tailed Student's t-test; *P< 0.05.