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. 2020 Dec 1;9:43. doi: 10.1186/s40035-020-00223-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — α-Synuclein particles and acidic compartments by light microscopy. a Deconvolved confocal images of live HEK293T cells transfected with A53T-α-synuclein (Syn)-EGFP and treated with lysotracker red for 2 h at 37 °C. Arrows show an aggresome. Note the distribution of lysotracker-positive vesicles close to the aggresome and other αSyn aggregates. b Deconvolved confocal image of HEK293T cells transfected with A53T-α-Syn-mCherry, the lysosomal marker LAMP1-EGFP and the cargo protein p62 without fluorescent tag. Enlarged insets show a cluster of LAMP1-decorated vesicles colocalizing with aSyn. Arrow shows a large aggresome, so called “p62 body”. c Deconvolved confocal image of HEK293T cells transfected with A53T-α-syn-EGFP and a biosensor of the autophagosome lipid phosphatidylinositol 3-phosphate (PI3P) tagged to mCherry. Enlarged insets show the PI3P-positive vesicles around the aggresome (arrow), consistent with the hypothesis that autophagosomes degrade aggresomes. In panels b-c, the red and green channels of the insets are shown individually next to the merged images. Scale bars, 5 μm. Original data