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. 2020 Dec 1;20:908. doi: 10.1186/s12879-020-05664-6

Table 1.

PCR primers used in the study

Primer Primers (5_ to 3_) Amplicon
size (bp)
Annealing temperature °C Reference
pCVD432

F-CTGGCGAAAGACTGTATCAT

R-CAATGTATAGAAATCCGCTGTT

630 58 [19]
eae

F-CTGAACGGCGATTACGCGAA

R-CCAGACGATACGATCCAG

917 58 [20]
bfpA

F-AATGGTGCTTGCGCTTGCTGC

R-GCCGCTTTATCCAACCTGGTA

326 58 [21]
LT gene

F-GGCGACAGATTATACCGTGC

R-CGGTCTCTATATTCCCTGTT

450 50 [22]
ST gene

F-ATTTTTMTTTCTGTATTRTCTT

R-CACCCGGTACARGCAGGATT

190 50 [22]
ipaH

F-GTTCCTTGACCGCCTTTCCGATACCGTC

R-GCCGGTCAGCCACCCTCTGAGAGTAC

600 52 [23]
stx1

F-ATAAATCGCCATTCGTTGACTAC

R-AGAACGCCCACTGAGATCATC

180 50 [24]
Stx2

F-GGCACTGTCTGAAACTGCTCC

R-TCGCCAGTTATCTGACATTCTG

255 50 [22]
ChuA

F- ACGAACCAACGGTCAGGAT

R-TGCCGCCAGTACCAAAGACA

279 59 [10]
yjaA

F-TGAAGTGTCAGGAGACGCTG

R-ATGGAGAATGCGTTCCTCAAC

211 59 [10]
TspE4C2

F-GAGTAATGTCGGGGCATTCA

R-CGCGCCAACAAAGTATTACG

154 59 [10]
blaTEM

F-AAACGCTGGTGAAAGTA

R-AGCGATCTGTCTAT

822 58 [25]
blaSHV

F-ATGCGTTATATTCGCCTGTG

R-TGCTTTGTTATTCGGGCCAA

753 60 [25]
CTX-M-15

F-CACACGTGGAATTTAGGGACT

R-GCCGTCTAAGGCCATAAACA

996 55 [26]
MOX

F-GCTGCTCAAGGAGCACAGGAT

R-CAC ATT GAC ATA GGT GTG GTG C

520
FOX

F-AAC ATG GGG TAT CAG GGA GAT G

R-CAA AGC GCG TAA CCG GAT TGG

190 64 [27]
DHA

F-AAC TTT CAC AGG TGT GCT GGG T

R-CCG TAC GCA TAC TGG CTT TGC

405
CIT

F-TGG CCA GAA CTG ACA GGC AAA

R-TTT CTC CTG AAC GTG GCT GGC

462