Fig. 5.

miR-29b-1-5p target 3′-UTR of RAP1B and regulates cellular function of WJ-MSCs. a 3′ UTR sequences containing the predicted “seed region” target site of RAP1B and its mut-sequences were cloned into the psiCHECK2.0 vector to prove whether RAP1B was the direct target gene of miR-29b-1-5p. b Dual-luciferase reporter gene assay to detect interaction between miR-29b-1-5p and RAP1B. RAP1B-mut, RAP1B 3′UTR mutant type; RAP1B-wt, RAP1B wild type; mimics NC, empty vector control. ^**P < 0.001. c, d qRT-PCR was used to determine the miR-29b-1-5p expression level of WJ-MSCs transfected with mimics/inhibitor. ^**P < 0.001 compared to mimics/inhibitor NC group. e qRT-PCR was used to determine the RAP1B mRNA expression level of WJ-MSCs in four groups. ^**P < 0.001. f Western blot assay was performed to verify whether miR-29b-1-5p affected the RAP1B protein level of WJ-MSCs. g Quantitative analysis of western blot assay. Data in panel are shown as means ± standard deviation (n = 3). ^*P < 0.05, ^**P < 0.001