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. 2020 Aug 5;22(12):2041–2051. doi: 10.1038/s41436-020-0915-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, and provide a link to the Creative Commons license. You do not have permission under this license to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — (a) Reverse-transcription polymerase chain reaction (RT-PCR) showing the expression of DYNC2H1 in BJ fibroblasts (fibs), induced pluripotent stem cells (iPSCs), and retinal organoids at 40, 90, 100, 120, 150, and 200 days of differentiation. The shorter PCR product on the gel corresponds to the canonical DYNC2H1 transcript from exons 63 and 65. The longer amplicon (*) includes the retinal-specific microexon 64 (human exon ID GT_21385 on http://ascot.cs.jhu.edu/). (b) Sequencing electropherograms of the canonical and retinal (*) isoforms with alignment to the DYNC2H1 reference sequence. The retinal microexon is boxed in red. (c) Retinal organoid differentiation was confirmed by RT-PCR for markers of retinal differentiation (PAX6, VSX2, CRX, NRL, NR2E3) at 40, 60, 80, 100, 150, and 200 days of differentiation.