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. 2020 Nov 27;13:12225–12241. doi: 10.2147/OTT.S281397

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© 2020 Li et al.

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Establishment of the co-culture system of Jurkat lymphocytic cell and HePG2 liver cancer cell. (A) The effect of IFN-γ on the expression of PD-L1 by HepG2 cell determined by Western blot. Densitometric values analyzed using the AlphaEaseFC 4.0 software program. β-Actin served as an internal control. Protein expression was normalized against the control. (B) The effect of PHA on morphology of Jurkat cell observed using an inverted microscope. (C) The cytotoxicity of PHA on Jurkat cells detected by Cell Counting-Kit 8. Cell viability was compared with control cells (100%). (D) PHA promotes the secretion of IL-2 by Jurkat cells. The data are presented as the mean ± S.D. (n=3), **p<0.01, ***p<0.001 vs control.