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. 2020 Nov 27;13:12225–12241. doi: 10.2147/OTT.S281397

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© 2020 Li et al.

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The expression of PTEN, p-Akt, Akt, p-mTOR, mTOR, Cleaved caspase-9, Cleaved caspase-3 and Bax in Jurkat cells determined by immunofluorescence staining. (A) Proteins stained by indirect fluorescence with secondary antibodies conjugated to Alexa Fluor-488 (green). Nuclei were counterstained with DAPI (blue). A representative image (n=3) is shown. Images were collected using a Leica TCS SP5 confocal imaging system. (B) The bar graphs show the fluorescence intensity of the proteins. The data are presented as the mean ± S.D. (n=10). *p<0.05, **p<0.01 vs Jurkat (PHA); ^##p<0.01 vs Jurkat (PHA)+HepG2 (IFN-γ).