Figure 1.

P2Et treatment modulates the expression of PD-L1 on B16-F10 and 4T1 cells. (A) Geometric Mean Fluorescence Intensity (MFI) of surface or intracellular PD-L1 expression in B16-F10 and 4T1 cells after 48 h. (B) PD-L1 relative expression to GAPDH gene in B16-F10 and 4T1 cultured cells during 48 h with one-half of the P2Et IC₅₀ extract, cobaltous chloride (CoCl₂) or ethanol (EtOH) analyzed by qRT-PCR and quantitated as a fold change against the control by using the 2^−ΔΔCT method. Representative histograms of surface and intracellular PD-L1 expression in (C) B16-F10 and (E) 4T1 cells treated during 48 h with one-half of the P2Et IC₅₀, CoCl₂ or ethanol. Geometric MFI of surface and intracellular PD-L1 expression in (D) B16-F10 and (F) 4T1 cells treated. In all cases data are represented as the mean ± SEM of at least three independent experiments. The p values were calculated using a Mann–Whitney U test or Kruskal–Wallis test with Dunn’s post-test. *p < 0.05, **p < 0.01, ****p < 0.0001.