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Type II NKT cells require CD1d to recognize total SA lipids. (A, B) IFN-γ (A) and IL-17A (B) ELISPOT of Jα18-/- total liver lymphocytes collected from 4 dpi livers and co-cultured with MHC-II-/- or MHC-II-/-CD1d-/- DCs, unpulsed or pulsed overnight with 10 µg/ml total SA lipids (SAlip) (N=4 mice/experiment, representative 1 of 3). Statistical analysis: (A, B) 2-way ANOVA.***p < 0.001; ****p < 0.0001.
