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. 2020 Dec 18;13(12):1854–1863. doi: 10.18240/ijo.2020.12.03

Figure 5. Mito Stress tests were performed on SSRGC-5 cells after narrow-band blue light exposure.

Figure 5

A: In the Mito Stress tests, each data point represents 3 wells, and cellular OCRs of SSRGC-5 cells in both control and BL group were measured. Arrows point to the injection of the electron transport chain inhibitors. Oligomycin is injected after 3 basal OCR readings; next FCCP, an uncoupler, is injected, which leads to an increase in OCR; this is followed by injection with both antimycin A and rotenone, leading to inhibition of complex I and complex III of the electron transport chain. All the OCR values in different time points (0, 1, 3, 6, 12, 18, 24h) were shown as two groups, control group and BL group. B: Baseline OCR comprises non-mitochondrial respiration, proton leak-linked, and ATP-related respiration. In BL group, baseline OCR showed significant decreases caused by ATP-related respiration in a time-dependent manner compared with control group. C: The basal respiration, ATP-linked respiration, maximal respiration and spare respiration capacity were compared between control group and BL group. aP<0.05.