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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Epilepsia. 2020 Feb 18;61(3):561–571. doi: 10.1111/epi.16445

Figure 2.

Figure 2.

Quantification of electrographic seizures in the IHKA model over time. Animals given IHKA showed both (A) electrographic seizures with no behavioral manifestation and (B) and behavioral seizures. Scale bars are (A) 1 mV, 5 sec, and (B) 2 mV, 5 sec. Asterisks in (A) indicate events classified as electrographic seizures. (C, D) Seizure duration and seizure frequency were quantified in a semi-automated manner in IHKA treated animals (n=4) over the course of 24 hours and no significant differences were found in (C) seizure frequency (p=0.34, Degrees of freedom (DF)=23, Kruskal-Wallis ANOVA followed by Dunn’s Test) but a significant difference was found within the (D) seizure duration (p<0.001, DF=23, 9 am seizure duration significantly different compared to 4 am, 5 am, 7 am, p<0.05, and 9 am seizure duration significantly different compared to 6 pm, 10 pm, 11 pm, p<0.01). Shaded areas indicate light-off phase of light cycle. Following this, seizure duration and seizure frequency were analyzed for one day a month over the course of 6 months (E, F) which was limited to the same 5–6 hour period of time each day, approximately 7 am until 12 pm, due to the fluctuations seen in (C, D). No significant changes were seen in seizure frequency (p=0.76, DF=5, Kruskal-Wallis ANOVA) and seizure duration (p=0.24, DF=5) in these animals (n=4) during this time.