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. 2020 Dec 1;10:20918. doi: 10.1038/s41598-020-77788-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PET analysis of ¹⁸F-mFBG distribution and NET-1 expression profile. (a) Representative coronal slice of Kelly (left) and SK-N-BE(2)C (right) xenograft PET images with corresponding maximum intensity projection CT overlay, 4 h p.i. of ¹⁸F-mFBG. Dotted circles represent tumour region. (b) PET quantification of Kelly and SK-N-BE(2)C xenografts 1 and 4 h p.i. of ¹⁸F-mFBG. Data are presented as mean ± SD, n ≥ 5 per group, each dot represents one tumour. ***p < 0.001, ****p < 0.0001; Student’s t-test. Ex vivo analysis of Kelly (c) and SK-N-BE(2)C (d) xenografts (i) Western blot (n = 2) (Full-length blots are presented in Supplementary Figure WB2) (ii) immunohistochemistry of NET-1 protein (× 40) and (iii) autoradiography exposition of tumour section, 2 h on to X-ray film. Graph is generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com.