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. 2020 Dec 1;10:20976. doi: 10.1038/s41598-020-77865-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum likelihood trees depicting the relationship of fungal isolates obtained in this study with other members of the Nannizziopsis genus for which similar sequences were available. Analyses were performed using (A) ITS2, (B) 28S, (C) β-tubulin, and (D) concatenated (ITS + 28S + β-tubulin) sequence alignments. Midpoint-rooted consensus trees were constructed from 750 original trees produced using PhyML, based on a generalized time reversible substitution model, bootstrapped with 1000 random re-samplings of the data. Branches are supported by consensus support threshold values > 75%. Scale bars represent the mean number of nucleotide substitutions per length.