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. 2020 Dec 1;10:20964. doi: 10.1038/s41598-020-78007-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transient overexpression of ScWRKY5 in Nicotiana benthamiana leaves. (A) Semi-quantitative PCR analysis of ScWRKY5 in N. benthamiana leaves inoculated with Agrobacterium GV3101 carrying pEarleyGate 203-ScWRKY5 (35S::ScWRKY5) and the empty vector pEarleyGate 203 (35S::00). (B) DAB staining analysis of N. benthamiana leaves after one day of inoculation. (C) Transcription levels of eight immune-related marker genes in N. benthamiana leaves after one day of inoculation. Data are normalized to the expression level of NtEF-1α. All data points are means ± standard error (n = 3). Bars superscripted by different lowercase letters indicate significant differences, as determined by Duncan’s new multiple range test (p-value < 0.05).