Fig. 4. Reconstruction examples for continuous volumetric fluorescent objects through a ground glass diffuser.

a–d Fluorescence images of different pollen seed structures taken after removing the diffuser. The blue line is a contour plot (at 10% of the maximum intensity) of the reconstructed object. e–h Reconstruction of the object with NMF+PR approach. The rank for the NMF is overestimated at r=80 for e–h and r=150 for f–g. Note that only the high variance eigen-patterns, are cross-correlated. In the four cases, P = 5120 fluorescent images are recorded at 50 Hz, N_SLM = 256 for e–g and N_SLM = 1024 for h.