Skip to main content
. 2020 Dec 1;3:726. doi: 10.1038/s42003-020-01454-7

Fig. 1. NPPs are still active in immature gametocytes.

Fig. 1

a Kinetics of sorbitol-induced isosmotic lysis of erythrocytes infected with early rings (4 h-post-invasion (hpi)), late rings (16 hpi), trophozoites (28 hpi), schizonts (40 hpi), stage II gametocytes and uninfected erythrocytes during 60 min. n = 3 independent experiments. b % lysis of stage II GIE in sorbitol at 60 min with or without 100 µM NPPB, Furosemide, Ro5-4864, or PK11195. c Lysis kinetics (left) and % lysis at 60 min (right) of stage II GIE in sorbitol, alanine or PhTMA+. d Patch-clamp experiments on erythrocytes infected with trophozoites, (green bar, number of cells = 14), stage II gametocytes (blue bar, number of cells = 14) or uninfected erythrocytes (red bar, number of cells = 11). Left: whole-cell conductance calculated at −100 mV. Right: I−V plot from patch-clamp experiments. e Patch-clamp experiments on stage II GIE in presence or absence of NPPB (number of cells = 14 and 5, respectively). Left: whole-cell conductance calculated at −100 mV. Right: I−V plot from patch-clamp experiments. f Viability (luciferase activity) of early gametocytes of the NF54-cg6-pfs16-CBG99 line 48 h after a 3-h incubation with or without 100 µM NPPB. The graph shows relative viability normalized by the average luciferase activity of control (without NPPB). a.u. arbitrary units. In (b, c, f), circles indicate the number of independent experiments. In (d, e), the data distribution is shown in Supplementary Fig. S1. Error bars show the standard error of the mean (SEM). Statistical significance is determined by a Mann−Whitney test (d−f) or by one-way ANOVA with Dunnet correction (b) or Sidak correction (c) for multiple comparisons. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns: non-significant difference.