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. 2020 Dec 1;3:726. doi: 10.1038/s42003-020-01454-7

Fig. 3. NPP activity is regulated by cAMP-signaling.

Fig. 3

a, b Sorbitol-induced isosmotic lysis of stage II GIE with 100 µM H89 or 10 µM KT5720 (a), or with 100 µM 8Br-cAMP (b). c Sorbitol-induced isosmotic lysis of stage II GIE from the NF54 isolate (Control) and the transgenic pHLpfpkar line, cultivated with or without pyrimethamine (Pyri), or preincubated with 100 µM 8Br-cAMP. d, e Sorbitol-induced isosmotic lysis of stage V GIE with 100 µM 8Br-cAMP (d), sildenafil (e) or tadalafil (e). f Sorbitol-induced isosmotic lysis of stage V gametocytes from the NF54 isolate and the transgenic line PDEδ−. All experiments were performed in the presence (orange) or absence (blue) of 100 µM NPPB. Circles indicate the number of independent experiments and error bars show the SEM. Statistical significance is determined by one-way ANOVA with Sidak correction for multiple comparisons. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns: non-significant difference.