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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Environ Pollut. 2020 Sep 4;267:115597. doi: 10.1016/j.envpol.2020.115597

Fig. 4. Upregulation of miR-21 in primary monocytes treated with Nano-Ni and Nano-Ni-P in vitro (A) and in vivo (B) by real-time PCR.

Fig. 4.

(A) Primary monocytes were isolated from peripheral blood of wild-type mice and treated with 30 μg/mL of Nano-Ni or with either Nano-Ni-P or Nano-Ni-C with same molar concentration of Ni as Nano-Ni for 24 h. Data are shown as mean ± SE (n=3). (B) Wild-type mice were intratracheally instilled with 50 μg per mouse of Nano-Ni or with either Nano-Ni-P or Nano-Ni-C with same molar concentration of Ni as Nano-Ni, and monocytes were isolated from peripheral blood at day 3 after exposure. Data are shown as mean ± SE (n=3~6). Cells or mice treated with physiological saline were used as the control. miR-21 expression was determined by real-time PCR and normalized by endogenous control U6 snRNA. * p<0.05 vs. Control; # p<0.05 vs. Nano-Ni or Nano-Ni-P group.