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(A) Staining for viability of hiPSCs cultured in 2D (I-III) and 3D (IV-VI) cultures. Representative fluorescence images showing the live (Calcein-AM, green) and dead (BOBO-3 iodide, red) cells in 2D and 3D cultures. Scale: Scale: 400 μm. (B) Lactate dehydrogenase (LDH) release measured in 2D and 3D cultures. n = 4, NS: *p > 0.05.
