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. 2020 Nov 27;12:12259–12267. doi: 10.2147/CMAR.S260866

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© 2020 Qi et al.

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GAS5 functions as a ceRNA of miR-21 in UM. (A) The binding site of miR-21 on GAS5. (B) miR-21 expression in UM cells after overexpression or knockdown of GAS5 was measured by qRT-PCR analysis. (C) Luciferase activity in C918 cells co-transfected with miR-21 mimics and luciferase reporters containing GAS5-WT or GAS5-MUT (*P<0.05). (D) Expression levels of miR-21 in UM tissues and normal uveal tissues were determined by qRT-PCR analysis. Data are shown as mean ± SD. (E) The correlation between miR-21 and GAS5 expression in UM tissues was determined.