Table 2.
Protective effects of various treatments against GO-induced ROS formation in rat renal cells.
| Treatment | ROS formation (fluorescence intensity) |
||
|---|---|---|---|
| 15 min | 30 min | 60 min | |
| Control isolated renal cells | 47 ± 3 | 55 ± 2 | 62 ± 4 |
| +GO (5 mM) | 62 ± 5a | 75 ± 3a | 95 ± 4a |
| +Resveratrol (50 μM) | 57 ± 3 | 62 ± 4b | 71 ± 3b |
| +Curcumin (5 μM) | 54 ± 5 | 56 ± 3b | 67 ± 5b |
| +Gallic acid (10 μM) | 51 ± 3b | 56 ± 5b | 73 ± 3b |
| +DMSO (150 mM) | 50 ± 5b | 61 ± 3b | 75 ± 6b |
| +Mannitol (50 mM) | 48 ± 4b | 63 ± 4b | 76 ± 4b |
| +Carnitine (2 mM) | 42 ± 3b | 59 ± 2b | 72 ± 5b |
| +L-Glutamine (1 mM) | 49 ± 3b | 67 ± 3b | 78 ± 5b |
| +Chloroquine (100 mM) | 54 ± 4 | 64 ± 5b | 81 ± 3b |
Data are expressed as mean ± SD of three separate experiments (n = 3).
Significantly different from the control renal cells (P < 0.05).
Significantly different from the GO-treated renal cells (P < 0.05). GO: glyoxal; ROS: reactive oxygen species; DMSO: dimethyl sulfoxide and SD: standard deviation.