Fig. 2.

Assessment of aflatoxin B1 toxicity on liver cells grown as 2D monolayers. (A) Monolayers of mature HepaRG cells, treated with DMSO, 10 μM AFB1, and 20 μM AFB1 at the indicated time points were tested for cell survival using live-dead staining. Live cells fluoresce green, whereas dead cells fluoresce red. Graphs are representative images of three independent experiments. (B) Metabolic activity of mature HepaRG cells grown in 2D upon AFB1 treatment at the indicated time points. Activities were determined using tetrazolium hydroxide salt (XTT) assays. Absorbance was carried out at 450 nm. (C) Albumin levels of mature HepaRG cells in 2D monolayers upon AFB1 exposure were quantified at the indicated time points using ELISA analysis. Results are shown as mean ± SD of three independent experiments. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.