Skip to main content
. 2020 Nov 21;7:1578–1587. doi: 10.1016/j.toxrep.2020.11.003

Fig. 3.

Fig. 3

Effect of aflatoxin B1 on liver cells in 3D printed constructs. (A) Cell viability of mature HepaRG in 3D constructs upon treatment with AFB1 (10 μM or 20 μM) for the indicated time periods was visualized using the live-dead staining; calcein-AM (living cells in green) and ethidium homodimer-1 (dead cells in red). (B) Number of green-stained cells quantified with ImageJ in constructs treated with AFB1 for the indicated time points. Bars indicate the means ± SD, n ≥ 3 images. (C) Metabolic activity of the mature HepaRG cells treated with DMSO or AFB1 (10 μM or 20 μM) inside the 3D printed alginate/gelatin/Matrigel constructs was determined by the XTT assay at the indicated time points. Absorbance was measured at 450 nm. (D) Levels of albumin secreted from mature HepaRG cells in 3D constructs upon DMSO or AFB1 (10 μM or 20 μM) treatment were quantified at the indicated time points using ELISA analysis. Results are shown as mean ± SD of three independent experiments. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.