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. 2020 Dec 1;10:20966. doi: 10.1038/s41598-020-77915-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic illustration of the perfusion loops for arterial PV (a) and venous PV groups (b). (a) In arterial PV group, arterial blood was provided in the PV with total PV flow rate of 1 l/min. For this purpose blood flow was split after the oxygenator into the PV and HA lines with the same oxygen content. (b) In venous PV group, venous blood was provided in the PV with total PV flow rate of 1 l/min. For this purpose, oxygen deprived blood from the liver output was mixed with oxygenated blood after oxygenator. Flow rates in the arterial and deoxygenated lines were automatically adjusted to maintain an oxygen saturation at the liver output/outlet of ~ 65%. (c) Representative illustration of the perfusion machine. HA hepatic artery, PV portal vein, VC vena cava.