Fig. 5.

Seed-based functional connectivity analysis. Regions where ALS patients showed increased (cold colors) or reduced (warm colors) functional connectivity with the left and right middle frontal gyrus (MFG) compared to healthy controls at baseline (A) and at follow-up (6 months) (B), accounting for changes at the ALS Functional Rating Scale Revised (ALSFRS-R) and time between scans. Results are overlaid on the Montreal Neurological Institute (MNI) standard brain and displayed at p < 0.05 Family-wise error corrected for multiple comparisons at a cluster level. X, y and z values denotes MNI coordinates. Colour bar represents Z values.