Fig. 2.

Lung morphology of wild-type (Gsr-WT) and glutathione reductase-deficient (Gsr-KO) mouse lungs from canalicular to matured stages. H&E-stained lungs from Gsr-KO mice (200x) display evidence of septal thickening and alveolar epithelial dysplasia compared to Gsr-WT at embryonic day E19 (A) and persistent septal thickening alveolar epithelial cell dysplasia at PND3 (B) which has essentially normalized by PND56 (C). Bars = 50 μm. Pulmonary function assessment in PND56 mice for pulmonary resistance (D), compliance (E), total lung capacity (F) and airway resistance (G) were assessed. Pulmonary resistance and total lung capacity were significantly decreased in Gsr-KO mice compared to Gsr-WT. (H) Methacholine responses reveal enhanced airway responsiveness in Gsr-KO compared to Gsr-WT at PND 56. Data are expressed as mean +SEM (*p < 0.05 vs Gsr-WT; n=14–18).