Fig. 4.

Effect of glutathione reductase (Gsr) deficiency on embryonic and postnatal lung transcriptomics. (A) Venn Diagram analysis determined Gsr-dependently expressed genes at embryonic day E19 and postnatal days 5 (PND5) and PND56. Common Gsr-dependent genes at different developmental ages are shown as same color/pattern blocks in the bar graphs. (B) At E19 (canalicular stage), lungs in Gsr-deficient (Gsr-KO) embryo displayed significantly lowered expression of genes for cell cycle control of chromosomal replication and DNA damage checkpoint regulation and heightened expression of inflammatory and cell death response genes. (C) At PND5 (entering alveolar stage), Gsr-KO lungs showed significantly suppressed transcriptome for lipid metabolism and transport and coagulation system. (D) In mature lung (PND56), Gsr deficiency modulated genes to promote abnormal cardiovascular system function and connective tissue disorders. Analyses were done using GeneSpring (moderated t-test followed by Benjamini-Hochberg multiple testing correction, p < 0.01) and Ingenuity Pathway Analysis software. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)