Table 1.
Representative glutathione reductase (Gsr)-dependent genes in embryo to adult mouse lungs.
| Category (Age) | RefSeq ID | †FD | Gene Symbol | Gene Title | Functions |
|---|---|---|---|---|---|
| Cell cycle, DNA damage checkpoint regulation (E19) | NM_001025779 | −3.14 | Cdc6 | cell division cycle 6 | DNA replication initiation |
| NM_007659 | −2.26 | Cdk1 | cyclin-dependent kinase 1 | protein complex assembly | |
| NM_001302540 | −2.66 | Mcm5 | minichromosome maintenance deficient 5 | DNA replication initiation | |
| NM_001291185 | −2.41 | Aurka | aurora kinase A | meiotic spindle organization | |
| NM_172301 | −2.36 | Ccnb1 | cyclin B1 | mitotic cell cycle | |
| NM_001195298 | −2.24 | Kifc1 | kinesin family member C1 | mitotic sister chromatid segregation | |
| Redox and metabolism (E19) | NM_029562 | −32.87 | Cyp2d26 | cytochrome P450, family 2, subfamily d, polypeptide 26 | xenobiotic metabolic process |
| NM_001039555 | −25.90 | Cyp2c68 | cytochrome P450, family 2, subfamily c, polypeptide 68 | xenobiotic metabolic process | |
| NM_019775 | −19.54 | Cpb2 | carboxypeptidase B2 (plasma) | fibrinolysis | |
| NM_001304800 | −18.34 | Hsd3b1 | hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 | lipid metabolism | |
| NM_001277944 | −15.45 | Apoc2 | apolipoprotein C-II | lipid metabolism | |
| NM_009467 | −12.76 | Ugt2b5 | UDP glucuronosyltransferase 2 family, polypeptide B5 | phase 2 conjugation | |
| NM_001305992 | −3.25 | Ggt1 | gamma-glutamyltransferase 1 | glutamate metabolic process | |
| NM_008185 | −2.88 | Gstt1 | glutathione S-transferase, theta 1 | glutathione metabolic process | |
| Inflammatory and immunological response (E19) | NM_009892 | 3.14 | Chil3 | chitinase-like 3 | polysaccharide catabolic process |
| NM_001164724 | 2.43 | Il33 | interleukin 33 | leukocyte migration | |
| NM_133871 | 3.66 | Ifi44 | interferon-induced protein 44 | immune response | |
| NM_008331 | 6.73 | Ifit1 | interferon-induced protein with tetratricopeptide repeats 1 | immune system process, response to virus | |
| NM_001099217 | 4.35 | Ly6c1 | lymphocyte antigen 6 complex, locus C1 | Activation of lymphocytes | |
| NM_022983 | 5.03 | Lpar3 | lysophosphatidic acid receptor 3 | activation of MAPK activity | |
| NM_144559 | 2.51 | Fcgr4 | Fc receptor, IgG, low affinity IV | NK T cell proliferation | |
| NM_021297 | 2.42 | Tlr4 | toll-like receptor 4 | innate immune response | |
| NM_016704 | 3.37 | C6 | complement component 6 | in utero embryonic development | |
| Coagulation system/Acute phase response signaling (PND5) | NM_175628 | −8.24 | A2m | alpha-2-macroglobulin | negative regulation of complement activation |
| NM_009244 | −21.46 | Serpina1b | serine (or cysteine) preptidase inhibitor, clade A, member 1 B | protein N-linked glycosylation | |
| NM_001111048 | −39.04 | Fga | fibrinogen alpha chain | adaptive immune response | |
| NM_008407 | −9.09 | Itih3 | inter-alpha trypsin inhibitor, heavy chain 3 | peptidase inhibitor activity | |
| NM_017371 | −4.13 | Hpx | hemopexin | positive regulation of immunoglobulin production | |
| NM_031164 | −6.00 | F13b | coagulation factor XIII, beta subunit | blood coagulation pathway | |
| NM_013697 | −23.5 | Ttr | transthyretin | retinol metabolic process, thyroid hormone transport | |
| Lipid metabolism (PND5) | NM_001277944 | −4.54 | Apoc2 | apolipoprotein C-II | lipid transport |
| NM_009692 | −16.65 | Apoa1 | apolipoprotein A-I | lipid transporter activity | |
| NM_009654 | −27.57 | Alb | albumin | fatty acid binding, transport | |
| NM_007443 | −8.44 | Ambp | alpha 1 microglobulin | protein-chromophore linkage | |
| Angiogenesis, connective tissue disorder (PND56) | NM_172399 | −2.54 | Ndnf | neuron-derived neurotrophic factor | angiogenesis |
| NM_054077 | −2.25 | Prelp | proline arginine-rich end leucine-rich repeat | axonogenesis | |
| NM_007734 | −1.87 | Col4a3 | collagen, type IV, alpha 3 | negative regulation of angiogenesis | |
| NM_009371 | −1.86 | Tgfbr2 | transforming growth factor, beta receptor II | patterning of blood vessels |
†Fold difference of baseline lung gene expression between Gsr-WT and Gsr-KO mice at the designated age (negative values indicate lowered expression in Gsr-KO than in Gsr-WT, positive values indicate heightened expression in Gsr-KO than in Gsr-WT). Full lists of the significantly varied genes between two genotypes determined by moderated t-test (p < 0.01) are in Supplementary Tables S1–S3. E=embryonic day. PND=postnatal day.