TABLE 2.

Effects of the probiotic mixture on junction protein (Tjp1, OCEL1, Cldn2) and inflammatory marker (TNF-α, Ifnγ, Il-10) RNAs in the ileum and colon of (A) Fischer and (B) maternally deprived Long Evans rats.

(A) Fischer rats
Ileum
	Tjp1	OCEL1	Cldn2	TNF-α	Ifnγ	Il-10
C	1.0 (0.5)	0.96 (0.3)	1.0 (0.3)	1.1 (0.9)	1.1 (0.6)	0.58 (1.2)
M	0.99 (0.4)	0.97 (0.4)	1.1 (0.7)	1.1 (0.7)	1.3 (1.2)	1.3 (0.9)
Colon
	Tjp1	OCEL1	Cldn2	TNF-α	Il-10
C	1.0 (0.3)	0.87 (0.6)	1.0 (0.5)	1.0 (0.7)	1.1 (0.9)
M	0.67 (0.3)***	0.81 (0.4)	0.47 (0.3)***	0.77 (0.6)	0.97 (0.7)
(B) Long Evans rats
Ileum
	Tjp1	OCEL1	Cldn2	TNF-α	Ifnγ	Il-10
NDC	0.99 (0.2)	0.93 (0.4)	0.99 (0.5)	1.02 (0.5)	0.86 (0.8)	1.06 (0.6)
NDM	0.85 (1.3)	1.14 (0.5)	1.19 (0.4)	0.86 (0.4)	1.59 (1.6)	0.79 (0.5)
DC	0.59 (0.2)***	1.11 (0.5)	1.03 (0.3)	1.12 (0.8)	2.90 (4.9)**	0.57 (0.7)
DM	0.88 (0.4)++	1.36 (0.5)	1.18 (0.5)	1.13 (0.8)	1.69 (4.8)	0.56 (0.7)
Colon
	Tjp1	OCEL1	Cldn2	TNF-α	Il-10
NDC	0.88 (0.4)	0.97 (0.3)	1.01 (0.6)	0.98 (1.1)	0.78 (0.7)
NDM	1.18 (0.7)*	1.42 (0.7)**	1.06 (1.1)	0.64 (0.8)	0.92 (1.0)
DC	0.94 (0.3)	1.33 (0.6)**	1.15 (0.7)	0.48 (0.3)	0.59 (0.4)
DM	1.15 (0.7)+	1.17 (1.2)	1.33 (0.6)	1.25 (1.1)	1.14 (1.2)

Results are expressed as 2^–ΔΔCt. Mann and Whitney test. n = 11–12. ***p = 0.001 vs. C. Mann and Whitney test n = 11–12, *p < 0.05, **p< 0.01, **p < 0.01 vs. NDC, +p < 0.05, ++p < 0.01 vs. DC.

ND, non deprived rats; D, deprived rats; C, control; M, probiotic mixture; Tjp1, tight junction protein 1; OCEL1, occludin 1; Cldn2, claudin 2; TNF-α, tumor necrosis factor; Ifnγ, interferon γ; Il-10, interleukine 10. The results are expressed as median and interquartile of 2^–ΔΔCt divided by the median of control group.