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. 2020 Sep 7;20(12):585–593. doi: 10.1002/elsc.202000032

FIGURE 3.

FIGURE 3

Schematic representation of Parvin and Snail knockout strategy based on homology arm directed cassette. The sgRNA‐targeting sequence is labeled in blue and protospacer‐adjacent motif (PAM) sequence are labeled in red; CBh, hybrid chicken β‐actinpromoter; EGFP, enhanced green fluorescence protein; β‐GpA, β‐globin polyA signal; BlaR, blasticidin‐resistance gene