Fig. 4. LIVAT-induced YAP nuclear entry decreased by SMG was not restored by daily LIVDT application.
MSCs were subjected to either CTRL, SMG, SMG + LIVDT over 72 h period were subsequently treated with LIVAT. Quantitative analysis of confocal images showed that SMG alone decreased basal nuclear YAP levels by 37%, which were increased back to control levels in the SMG + LIVDT group. +LIVAT increased nuclear YAP levels in the CTRL, SMG, and SMG + LIVDT groups by 50%, 69%, and 22%, respectively. Nuclear YAP intensity in the SMG + LIVDT + LIVAT group remained not significantly different from the SMG + LIVAT and 23% smaller than the LIVAT group. n > 200/grp. Group comparisons were made via Kruskal–Wallis test followed by Tukey multiple comparison, ****p < 0.0001. Error bars represent standard deviation. Scale bar: 10 μm. Full statistical details were provided in Supplementary Table 6.