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. 2020 Dec 1;6:35. doi: 10.1038/s41526-020-00125-5

Fig. 5. LPA treatment increases nuclear YAP levels.

Fig. 5

a Representative confocal images of DAPI (blue) and YAP (red) stained MSCs with or without LPA treatment. MSCs were subjected to LPA addition at 50 and 100 µM concentrations. b Quantitative analysis of confocal images revealed a 99% and a 107% increase in the 50 µM LPA and 100 µM LPA treatments compared to DMSO-treated controls, respectively. Nuclear YAP levels for 50 µM LPA and 100 µM LPA treatments were not significantly different. n > 30/grp. Group comparisons were made via Kruskal–Wallis test followed by Tukey multiple comparison, ****p < 0.0001. Error bars represent standard deviation. Scale bar: 10 μm. Full statistical details were provided in Supplementary Table 7.