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. 2020 Dec 1;10:20940. doi: 10.1038/s41598-020-77039-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Eucalyptus oil (Eu) and 1,8-cineole on inflammatory chemokine and cytokine production by bone-marrow-derived mast cells (BMMCs). (a) Histamine release was determined after treating anti-DNP-IgE-sensitised BMMCs with Eucalyptus oil and 1,8-cineole. Histamine release in the supernatant was specifically after 30 min of stimulation with DNP-human serum albumin (HSA) via ELISA. Histamine release was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (**P < 0.01 versus vehicle-DNP-HSA). (b,c) Production of IL-4 and IL-13 by anti-DNP-IgE-sensitised BMMCs was determined after treatment with Eucalyptus oil or 1,8-cineole. ELISA was performed after culturing cells with DNP-HSA for 3 h. IL-4 and IL-13 production was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (**P < 0.01 versus vehicle-DNP-HSA). (d,e) Production of prostaglandin D2 and leukotriene C4 by anti-DNP-IgE-sensitised BMMCs was determined after treatment with Eucalyptus oil or 1,8-cineole. ELISA was performed after culturing cells with DNP-HSA for 1 h. Prostaglandin D2 and leukotriene C4 production was calculated relative to that in cells treated with vehicle (0.1% DMSO) and stimulated with DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (**P < 0.01) of differences between vehicle- and DNP-HSA-treated cells. Dunnett’s test was used to assess the statistical significance (*P < 0.05, **P < 0.01 versus vehicle-DNP-HSA).