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. 2020 Nov 20;11(11):1373. doi: 10.3390/genes11111373

Table 3.

Analysis of molecular variance (AMOVA), based on 1023 permutations, for 24 accessions without grouping, and for 21 accessions by grouping them according to altitudinal range or regions of origin.

Source of Variation DF Sum of Squares Variance Components Percentage of Variation Fixation Indices Probability (p) Value
Among accessions 23 1078.2 1.019 Va 4.52 FST = 0.045 Va & FST < 0.0001
Among individuals within accessions 259 5921.0 1.321 Vb 5.86 FIS = 0.061 Vb & FIS < 0.0001
Within individuals 283 5722.0 20.219 Vc 89.63 FIT = 0.104 Vc & FIT < 0.0001
Total 565 12,721.3 22.556
a Among alt groups 2 97.9 0.026 Va 0.12 FCT = 0.001 Va & FCT = 0.1935
Among accessions within alt groups 18 800.1 1.015 Vb 4.67 FSC = 0.047 Vb & FSC < 0.0001
Within accessions 471 9746.7 20.694 Vc 95.21 FST = 0.048 Vc & FST < 0.0001
Total 491 10,644.8 21.735
b Among regions-I 5 217.0 −0.027 Va −0.12 FCT = −0.001 Va & FCT = 0.6715
Among accessions within regions-I 15 681.0 1.056 Vb 4.86 FSC = 0.048 Vb & FSC < 0.0001
Within accessions 471 9746.7 20.694 Vc 95.26 FST = 0.047 Vc & FST < 0.0001
Total 491 10,644.8 21.723
c Among regions-II 1 55.4 0.044 Va 0.20 FCT = 0.002 Va & FCT = 0.047
Among accessions within regions-II 19 842.6 1.011 Vb 4.65 FSC = 0.047 Vb & FSC < 0.0001
Within accessions 471 9746.7 20.694 Vc 95.15 FST = 0.048 Vc & FST < 0.0001
Total 491 10,644.8 21.723

Note: 21 of the 24 accessions were grouped according to region or altitudinal (alt) range or origin. The two cultivars and the breeding population were excluded from the grouping, as they cannot be placed in any of the groups. a The 21 accessions were grouped into three altitudinal groups: 1400–1680 m above sea level (masl), 1820–1968 masl, and 2045–2590 masl. b The 21 accessions were grouped into six regions (regions-I), and c the 21 accessions were grouped into two regions (regions-II) (Table S1).