Table 3.
Multivariable lineal regression analysis with LINE1 methylation as dependent variables in CRC patients.
| Variables | Both Sexes | Men Only | ||
|---|---|---|---|---|
| Low Vitamin B12 β (SE) |
High Vitamin B12 β (SE) |
Low Vitamin B12 β (SE) |
High Vitamin B12 β (SE) |
|
| LINE1 methylation: PBMCs | r2 = 0.32; p = 0.07 | r2 = −0.10; p = 0.99 | r2 = 0.38; p = 0.17 | r2 = −0.30; p = 0.51 |
| Age | 0.09 (0.04) | −0.00 (0.03) | 0.22 (0.04) | −0.08 (0.05) |
| Sex | −3.48 (1.16) * | −0.11 (0.71) | NA | NA |
| BMI | 0.05 (0.15) | 0.00 (0.08) | 0.00 (0.13) | 0.11 (0.18) |
| Neoadjuvant therapy | −0.07 (1.01) | −0.16 (0.78) | 0.13 (0.86) | −0.15 (1.67) |
| LINE1 methylation: tumor area | r2 = 0.51; p = 0.03 * | r2 = 0.18; p = 0.01 * | r2 = 0.64; p = 0.03 * | r2 = 0.32; p = 0.46 |
| Age | 0.05 (0.10) | 0.03 (0.07) | 0.07 (0.12) | 0.12 (0.16) |
| Sex | 2.63 (2.80) | −0.57 (1.65) | NA | NA |
| BMI | −0.85 (0.39) | 0.16 (0.18) | −0.88 (0.43) | −1.53 (1.61) |
| Neoadjuvant therapy | 7.80 (1.99) * | 5.99 (1.87) * | 7.87 (2.12) * | 8.92 (5.91) |
Lineal regression analysis with LINE1 methylation in PBMCs, VAT and tumor area as dependent variable and neoadjuvant therapy as independent variables and corrected for age, sex and BMI. Low and high vitamin B12 groups are divided under the 25th percentile of vitamin B12. Data are expressed as β (SE)of methylation (%). Asterisk indicates significant difference between low and high vitamin B12 groups, according to the linear regression analysis (* p < 0.05). Abbreviations: CRC, Colorectal cancer; PBMCs, peripheral blood mononuclear cell; LINE1, Long interspersed nuclear element-1; SE, Standard error; BMI, body mass index; r2: coefficient of determination; NA, not applicable.