Table 1.
The antiproliferative activity of IL3RA-ADC in a panel of tumor cells.
| Cell Line | Provider (Catalog No.) |
Date Obtained | Date Authenticated | Origin | Anti-IL3RA ABC |
IL3RA-ADC IC50 (M) |
|---|---|---|---|---|---|---|
| MV-4-11 | ATCC (CRL 9591) |
5/5/2008 | 02/05/2019 | Biphenotypic B myelomonocytic leukemia | ≈26,700 | 1.58 × 10−10 |
| MOLM-13 | DSMZ (ACC 554) |
5/2/2008 | 02/05/2019 | Acute myeloid leukemia | ≈15,100 a | 6.37 × 10−10 |
| HDLM-2 | DSMZ (ACC 17) |
19/02/2015 | 06/05/2015 | Pleural effusion of Hodgkin lymphoma | ≈74,300 | 1.97 × 10−9 |
| L-428 | origin unknown |
1996 | 17/04/2013 | Pleural effusion of Hodgkin lymphoma | ≈111,300 | 3.97 × 10−10 |
| THP-1 | ATCC (TIB 202) |
15/02/2006 | 19/03/2014 | Acute monocytic leukemia | ≈21,100 | 2.92 × 10−9 |
| KG-1 | ATCC (CCL 246) |
28/10/2010 | 24/03/2011 | Acute myelogenous leukemia | ≈7200 | 8.34 × 10−9 |
| HT | DSMZ (ACC567) |
12/09/2013 | 19/03/2014 | Diffuse mixed lymphoma | ≈350 | >3.00 × 10−7 |
| NCI-H292 | ATCC (CRL 1848) |
13/08/2009 | 07/02/2012 | Non-small cell lung cancer | ≈500 | >3.00 × 10−7 |
| HBL-1 | Charité (Prof. Lenz) | 15/04/2011 | 03/11/2017 | Diffuse B cell lymphoma | n.d. | n.d. |
| Kasumi-3 | DSMZ (ACC 714) |
20/04/2017 | 05/09/2017 | Acute myeloid leukemia | 23,500 | 6.89 × 10−9 b |
| Rec-1 | ATCC (CRL-3004) |
24/02/2014 | 23/08/2018 | Mantle cell lymphoma | n.d. | 1.03 × 10−7 |
| OVCAR-8 | NCI (NCI-60 panel) |
20/10/2008 | 02/05/2019 | Ovarian cancer | n.d. | 1.47 × 10−7 |
| MDA-MB-231 | ATCC HTB-26 |
05/04/2006 | 15/10/2019 | Breast cancer | ≈890 | >3.00 × 10−7 |
| Ramos | ATCC CRL 1596 |
08/03/2011 | 06/0572015 | Burkitt’s lymphoma | n.d. a | n.d. |
In vitro cytotoxicity (CellTiter-Glo®, Promega) of the IL3RA-ADC BAY-943 in cancer cell lines with different levels of anti-IL3RA antibody bound per cell (ABC) as determined by quantitative flow cytometry. The mean IC50 values from up to six individual assays are shown. n.d., not determined. a IL3RA expression analyzed by IHC on paraffin-embedded cell pellets; b IC50 determined at 144 h (at 72 h for the other cell lines).