Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 23;9(4):50. doi: 10.3390/antib9040050

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Biochemical and biophysical characterization of the hinge deleted IgG1 and its corresponding full-length control mAb by (a) SEC-UPLC coupled to UV (A280) and MALS. The UV elution profiles of the hinge deleted IgG1 molecule (bottom trace) and the corresponding full-length control (top trace) are shown. Molecular masses of 140 and 150 kDa were measured by MALS for the hinge deleted molecule and its full-length counterpart. (b) Nonreduced CE-SDS coupled to UV (A280). Electropherograms of the buffer control (bottom trace), hinge deleted IgG1 molecule (middle trace) and the corresponding full-length control (top trace) are shown. (c) Differential Scanning Fluorimetry. An overlay of the melting profiles of the hinge deleted IgG1 molecule (orange) and the corresponding full-length control (teal) are shown. Melting temperatures/onset were 67.8/56.5 °C for the hinge deleted molecule and 71.1/63.2 °C for the full-length control. (d) Intact reduced LC–MS. Total Ion Counts of the hinge deleted IgG1 molecule heavy chain (top trace) and the corresponding heavy chain of the full-length control (bottom trace) are shown. The peak corresponding to the dominant Fc N-Glycan G0F and G1F species are shown.