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AF4 fractograms of 20K and 106K pellets from sonicated platelets. Protein peak #1 and vesicle peak #2 are marked with rectangles. The strong UV absorbance at short retention times and overlapping the void volume is attributed to eluting proteins separated from the original centrifuged precipitates. AF4 elution is reversed relative to size exclusion chromatography with smaller particles eluting faster. RMS is the root-mean-square radius. Rh is the hydrodynamic radius.
